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TUDCA inhibits viral protein expression. A, quantitative RT-PCR of M1 and NP genomic viral RNA 12 h.p.i of control and TUDCA-pretreated cells. Results are shown as averages with S.E. B, Western blot analysis of influenza NP, NS1, and M1 proteins with corresponding densitometry displayed as averages with S.E. Proteins were isolated from independent experiments with similar groups as in A at 6 h.p.i.

Journal: The Journal of Biological Chemistry

Article Title: Influenza A Viral Replication Is Blocked by Inhibition of the Inositol-requiring Enzyme 1 (IRE1) Stress Pathway *

doi: 10.1074/jbc.M111.284695

Figure Lengend Snippet: TUDCA inhibits viral protein expression. A, quantitative RT-PCR of M1 and NP genomic viral RNA 12 h.p.i of control and TUDCA-pretreated cells. Results are shown as averages with S.E. B, Western blot analysis of influenza NP, NS1, and M1 proteins with corresponding densitometry displayed as averages with S.E. Proteins were isolated from independent experiments with similar groups as in A at 6 h.p.i.

Article Snippet: Influenza M1 mouse Ab (MA1–34775) was obtained from Thermo Scientific (Rockford, IL).

Techniques: Expressing, Quantitative RT-PCR, Western Blot, Isolation